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2026-10-16
CDC Identity Markers — Flow Cytometry
Tiered marker candidates for a cardiosphere-derived-cell (CDC) identity assay, with literature flow-antibody reagents.
CDCidentity assayflow cytometrysurface markersDESeq2
From the DESeq2 identity analysis (CDC vs 8 comparator cell types, 81 samples) we ranked every reliably-detected marker for a flow-cytometry identity assay on four axes — CDC-specificity, cell-surface accessibility, lot-to-lot reliability, and antibody availability — into three tiers. Tier 1 = prime surface candidates; Tier 2 = surface backups; Tier 3 = intracellular / lower-surface-evidence markers (require fix/perm for flow). For every marker we mined PubMed + full text for the antibodies actually used to detect it by flow cytometry and flagged which are literature-confirmed. Click any PMID to open the paper in the reader.
Tier 1 — prime surface candidates · 10 marker(s)High CDC-specificity, strong surface evidence, consistent across lots, antibody available. Start here.
Established — ready to order (3)
L1CAMCD171Neural cell adhesion molecule L1
surface 4/4CDC-specific 7/859 counts97% of lots
Established — multiple confirmed flow clones in the literature
| Clone | Fluor | Vendor | Cat # | Staining | Papers |
|---|---|---|---|---|---|
| 014 | — | — | — | unspecified | 27419370↗ |
| 14.10 | — | Biolegend | — | unspecified | 40805143↗ |
| 5G3 | — | Santa Cruz Biotechnology | sc-33686 | surface | 37943774↗ |
| 5G3 | PE | Abcam | — | unspecified | 35720083↗ |
| A10-A3 | — | — | — | unspecified | 28535665↗ |
| Ab417 | FITC | Sigma | — | surface | 28535665↗ |
27 supporting paper(s)
PMID 27419370↗PMID 28535665↗PMID 31500597↗PMID 31952346↗PMID 33897875↗PMID 34811658↗PMID 34812989↗PMID 35720083↗PMID 36337342↗PMID 37140802↗PMID 37943774↗PMID 37994665↗PMID 38306929↗PMID 38379293↗PMID 38678012↗PMID 40289262↗PMID 40473465↗PMID 40584698↗PMID 40669201↗PMID 40805143↗PMID 41437289↗PMID 41596610↗PMID 41795614↗PMID 41946147↗PMID 41996739↗PMID 42625444↗PMID 42827984↗
SLC3A2CD98Amino acid transporter heavy chain SLC3A2
surface 4/4CDC-specific 8/811,266 counts100% of lots
Established — multiple confirmed flow clones in the literature
| Clone | Fluor | Vendor | Cat # | Staining | Papers |
|---|---|---|---|---|---|
| B3 | — | — | — | surface | 10897033↗ |
| C-20 | — | Santa Cruz Biotechnology | — | unspecified | 35517419↗ |
| ECTO | FITC-conjugated anti-mouse secondary antibody | — | — | surface | 42350421↗ |
| REA861 | PE | Miltenyi Biotec | — | surface | 35920332↗ |
| UM7F8 | BV421 | BD Biosciences | — | surface | 39219024↗ |
| UM7F8 | PE | BD Biosciences | — | surface | 36462403↗ |
29 supporting paper(s)
PMID 10495377↗PMID 10731692↗PMID 10897033↗PMID 12392323↗PMID 21243931↗PMID 22170508↗PMID 23441163↗PMID 24025751↗PMID 24359579↗PMID 24615503↗PMID 28948414↗PMID 29399160↗PMID 30211967↗PMID 30592731↗PMID 31269269↗PMID 31601268↗PMID 34294905↗PMID 35517419↗PMID 35596131↗PMID 35920332↗PMID 36214569↗PMID 36462403↗PMID 39219024↗PMID 39744575↗PMID 40083086↗PMID 40999970↗PMID 41325364↗PMID 42213640↗PMID 42350421↗
DSG2Desmoglein-2
surface 4/4CDC-specific 7/881 counts92% of lots
Established — multiple confirmed flow clones in the literature
| Clone | Fluor | Vendor | Cat # | Staining | Papers |
|---|---|---|---|---|---|
| 141409 | Alexa Fluor 488 | R&D Systems | — | surface | 34245117↗ |
| 6D8 | — | Invitrogen | — | surface | 34245117↗ |
| 9F6 | — | — | — | surface | 34245117↗ |
| CSTEM28 | Alexa Fluor 488 | Invitrogen | — | surface | 34245117↗ |
2 supporting paper(s)
Usable — one confirmed clone (2)
CSPG4Chondroitin sulfate proteoglycan 4
surface 4/4CDC-specific 7/83,212 counts100% of lots
Usable — one confirmed flow clone; verify before use
26 supporting paper(s)
PMID 16309171↗PMID 22467596↗PMID 23124902↗PMID 25336400↗PMID 26002762↗PMID 26063918↗PMID 26560317↗PMID 26735180↗PMID 27678158↗PMID 27725773↗PMID 28376684↗PMID 28943635↗PMID 28964848↗PMID 29359814↗PMID 31021652↗PMID 32313730↗PMID 32900797↗PMID 33135273↗PMID 33918235↗PMID 34465515↗PMID 34638301↗PMID 35048331↗PMID 35625703↗PMID 37120719↗PMID 40650767↗PMID 41154668↗
CDH15Cadherin-15
surface 4/4CDC-specific 8/8249 counts100% of lots
Usable — one confirmed flow clone; verify before use
4 supporting paper(s)
Emerging — flow literature found, clone unverified (2)
CADM1 · surf 4/4PANX2 · surf 3/4
No flow literature — custom antibody needed (3)
LPAR3 · surf 3/4SLC38A5 · surf 3/4RHCG · surf 3/4
Tier 2 — surface backups · 54 marker(s)CDC-specific with some surface evidence — fallbacks if a Tier-1 marker fails validation.
Established — ready to order (10)
TNFRSF9CD137Tumor necrosis factor receptor superfamily member 9
surface 4/4CDC-specific 8/876 counts87% of lots
Established — multiple confirmed flow clones in the literature
| Clone | Fluor | Vendor | Cat # | Staining | Papers |
|---|---|---|---|---|---|
| 4B4-1 | PE | BD Biosciences | — | surface | 36932395↗38619623↗ |
| 4-1BB (CD137) | — | Bio X Cell | BP0296 | unspecified | 36927529↗ |
| 4B4-1 | APC | BD Biosciences | 550890 | surface | 37114204↗ |
| 4B4-1 | FITC | BioLegend | 309806 | surface | 34580116↗ |
| CD137 | — | — | — | unspecified | 40254392↗ |
21 supporting paper(s)
SLC7A5Large neutral amino acids transporter small subunit 1
surface 4/4CDC-specific 6/87,199 counts100% of lots
Established — multiple confirmed flow clones in the literature
PODXLPodocalyxin
surface 4/4CDC-specific 6/84,668 counts100% of lots
Established — multiple confirmed flow clones in the literature
TLR4CD284Toll-like receptor 4
surface 4/4CDC-specific 6/8759 counts100% of lots
Established — multiple confirmed flow clones in the literature
CD274CD274Programmed cell death 1 ligand 1
surface 4/4CDC-specific 6/8322 counts100% of lots
Established — multiple confirmed flow clones in the literature
| Clone | Fluor | Vendor | Cat # | Staining | Papers |
|---|---|---|---|---|---|
| 10F.9G2 | — | Bioxcell | BX-BE0101 | surface | 42151126↗ |
| 10F.9G2 | — | GeneTex | — | surface | 29250533↗ |
| 28-8 | — | ABCAM | — | surface | 37536063↗ |
| 28-8 | FITC | Abcam | ab224027 | surface | 37868689↗ |
| B7-H1 | — | E-bioscience | — | surface | 29898687↗ |
29 supporting paper(s)
PMID 26093818↗PMID 29250533↗PMID 29898687↗PMID 30042403↗PMID 31727135↗PMID 32237066↗PMID 32313717↗PMID 32334621↗PMID 33458446↗PMID 34439164↗PMID 34572014↗PMID 34800294↗PMID 35090328↗PMID 35296335↗PMID 35370422↗PMID 35530130↗PMID 35954195↗PMID 36628329↗PMID 36978108↗PMID 37536063↗PMID 37868689↗PMID 38093288↗PMID 39815258↗PMID 40180451↗PMID 40550561↗PMID 41033440↗PMID 41050650↗PMID 42151126↗PMID 42222594↗
ITGB2CD18Integrin beta-2
surface 4/4CDC-specific 6/823 counts57% of lots
Established — multiple confirmed flow clones in the literature
| Clone | Fluor | Vendor | Cat # | Staining | Papers |
|---|---|---|---|---|---|
| 327C | — | — | — | unspecified | 22701459↗ |
| IB4 | — | — | — | unspecified | 28445705↗ |
| Mac-1 | — | — | — | surface | 21356739↗ |
| mAb 24 | — | — | — | unspecified | 22701459↗ |
20 supporting paper(s)
CLDN1Claudin-1
surface 3/4CDC-specific 7/871 counts78% of lots
Established — multiple confirmed flow clones in the literature
| Clone | Fluor | Vendor | Cat # | Staining | Papers |
|---|---|---|---|---|---|
| 6F6 | — | — | — | surface | 28659146↗ |
| JAY-8 | — | Zymed laboratories Inc | — | surface | 28659146↗ |
2 supporting paper(s)
KDRCD309Vascular endothelial growth factor receptor 2
surface 3/4CDC-specific 6/8496 counts100% of lots
Established — multiple confirmed flow clones in the literature
ITGA4CD49dIntegrin alpha-4
surface 3/4CDC-specific 6/8358 counts100% of lots
Established — multiple confirmed flow clones in the literature
PDPNPodoplanin
surface 2/4CDC-specific 6/8318 counts100% of lots
Established — multiple confirmed flow clones in the literature
| Clone | Fluor | Vendor | Cat # | Staining | Papers |
|---|---|---|---|---|---|
| SZ168 | — | — | — | unspecified | 31208371↗42272990↗ |
| 18H5 | — | Abcam | — | surface | 31208371↗ |
| 8.1.1 | — | — | — | surface | 38561690↗ |
| 8.1.1 | APC | Biolegend | — | surface | 25849579↗ |
| 8.1.1 | PE | EBioscience | — | surface | 31083674↗ |
| D2-40 | — | — | — | unspecified | 25629270↗ |
23 supporting paper(s)
PMID 23455181↗PMID 25629270↗PMID 25849579↗PMID 28122639↗PMID 31083674↗PMID 31208371↗PMID 31702112↗PMID 31966841↗PMID 32668112↗PMID 33022771↗PMID 33982015↗PMID 34161160↗PMID 34310368↗PMID 34844926↗PMID 35304101↗PMID 37146034↗PMID 38561690↗PMID 38989462↗PMID 39594582↗PMID 41527005↗PMID 41684157↗PMID 42272990↗PMID 42689324↗
Usable — one confirmed clone (3)
F2RL3Proteinase-activated receptor 4
surface 4/4CDC-specific 7/8155 counts87% of lots
Usable — one confirmed flow clone; verify before use
2 supporting paper(s)
LAMP3CD208Lysosome-associated membrane glycoprotein 3
surface 4/4CDC-specific 6/8202 counts93% of lots
Usable — one confirmed flow clone; verify before use
5 supporting paper(s)
SLAMF9SLAM family member 9
surface 2/4CDC-specific 7/822 counts62% of lots
Usable — one confirmed flow clone; verify before use
2 supporting paper(s)
Emerging — flow literature found, clone unverified (12)
SEMA7A · CD108 · surf 4/4SLC29A1 · surf 4/4PCDH1 · surf 4/4DNER · surf 4/4IGDCC4 · surf 4/4GIPR · surf 3/4CLEC14A · surf 3/4SLC26A2 · surf 3/4SLC22A4 · surf 3/4SLC22A3 · surf 3/4CLDN14 · surf 2/4OSCAR · surf 2/4
No flow literature — custom antibody needed (29)
RTN4R · surf 4/4SLC39A14 · surf 4/4SLC38A1 · surf 4/4CDH4 · surf 4/4ADGRG6 · surf 4/4MFAP3L · surf 4/4EPHB1 · surf 4/4LRRC38 · surf 3/4NKAIN1 · surf 3/4ADAM19 · surf 3/4TMEM106A · surf 3/4SCN9A · surf 3/4IGSF3 · surf 3/4LTK · surf 3/4TGFA · surf 3/4NIPAL3 · surf 2/4P2RX5 · surf 2/4SEL1L · surf 2/4SLC17A9 · surf 2/4ATP10A · surf 2/4SLC37A2 · surf 2/4PLD5 · surf 2/4SELENOK · surf 2/4C2CD2L · surf 2/4CNIH3 · surf 2/4SLC46A3 · surf 2/4C3orf52 · surf 2/4TMEM233 · surf 2/4VSIG10L · surf 2/4
Tier 3 — intracellular / lower surface evidence · 239 marker(s)CDC-specific but little/no surface evidence — require fixation & permeabilization for flow.
Established — ready to order (1)
Usable — one confirmed clone (10)
TAP1Antigen peptide transporter 1
surface 1/4CDC-specific 8/81,223 counts100% of lots
Usable — one confirmed flow clone; verify before use
2 supporting paper(s)
CCL5C-C motif chemokine 5
surface 1/4CDC-specific 8/8111 counts90% of lots
Usable — one confirmed flow clone; verify before use
6 supporting paper(s)
TNFSF9Tumor necrosis factor ligand superfamily member 9
surface 0/4CDC-specific 8/8201 counts100% of lots
Usable — one confirmed flow clone; verify before use
LCP1Plastin-2
surface 0/4CDC-specific 7/859 counts83% of lots
Usable — one confirmed flow clone; verify before use
2 supporting paper(s)
KRT81Keratin, type II cuticular Hb1
surface 0/4CDC-specific 7/856 counts85% of lots
Usable — one confirmed flow clone; verify before use
2 supporting paper(s)
SYT13Synaptotagmin-13
surface 0/4CDC-specific 7/842 counts67% of lots
Usable — one confirmed flow clone; verify before use
1 supporting paper(s)
PDIA4Protein disulfide-isomerase A4
surface 0/4CDC-specific 6/813,697 counts100% of lots
Usable — one confirmed flow clone; verify before use
1 supporting paper(s)
KRT7Keratin, type II cytoskeletal 7
surface 0/4CDC-specific 6/812,088 counts100% of lots
Usable — one confirmed flow clone; verify before use
3 supporting paper(s)
TSC22D3TSC22 domain family protein 3
surface 0/4CDC-specific 6/8941 counts100% of lots
Usable — one confirmed flow clone; verify before use
2 supporting paper(s)
FAM167AProtein FAM167A
surface 0/4CDC-specific 6/8638 counts100% of lots
Usable — one confirmed flow clone; verify before use
1 supporting paper(s)
Emerging — flow literature found, clone unverified (122)
ANGPTL4 · surf 1/4HERPUD1 · surf 1/4CRELD2 · surf 1/4IFI6 · surf 1/4HERC6 · surf 1/4DERL3 · surf 1/4PIGA · surf 1/4UCP2 · surf 1/4SYVN1 · surf 1/4LIPG · surf 1/4CAMK4 · surf 1/4FAM241B · surf 1/4SERPINA1 · surf 1/4ITPR3 · surf 1/4IFI27 · surf 1/4SERP1 · surf 1/4AIFM2 · surf 1/4ROBO4 · surf 1/4EPSTI1 · surf 1/4INHBE · surf 1/4RETREG1 · surf 1/4HSPA5 · surf 0/4HYOU1 · surf 0/4PPP1R15A · surf 0/4ISG15 · surf 0/4MX1 · surf 0/4ATF3 · surf 0/4XAF1 · surf 0/4B4GALNT1 · surf 0/4IFIT1 · surf 0/4ZFAND2A · surf 0/4HES4 · surf 0/4TRIM14 · surf 0/4BHLHA15 · surf 0/4CDA · surf 0/4SH2D2A · surf 0/4CSF2 · surf 0/4PKP3 · surf 0/4TNNT1 · surf 0/4HSPA6 · surf 0/4NCF2 · surf 0/4RSAD2 · surf 0/4SERPINE1 · surf 0/4HSP90B1 · surf 0/4RND3 · surf 0/4MSC · surf 0/4FBXO32 · surf 0/4DNAJC3 · surf 0/4FOXD1 · surf 0/4OAS3 · surf 0/4DDIT3 · surf 0/4CXCL2 · surf 0/4TYMP · surf 0/4IRF7 · surf 0/4GDNF · surf 0/4CARD11 · surf 0/4SP110 · surf 0/4IFI44L · surf 0/4OAS2 · surf 0/4NGF · surf 0/4GCLC · surf 0/4OAS1 · surf 0/4CXCL5 · surf 0/4SIM2 · surf 0/4IFI44 · surf 0/4STPG1 · surf 0/4RCAN3 · surf 0/4LINC01705 · surf 0/4COL4A3 · surf 0/4CALB2 · surf 0/4NKX3-1 · surf 0/4STOX2 · surf 0/4APC2 · surf 0/4MYB · surf 0/4MAD1L1 · surf 0/4COL4A1 · surf 0/4SQSTM1 · surf 0/4CXCL8 · surf 0/4RALA · surf 0/4PLIN2 · surf 0/4IL32 · surf 0/4IL1B · surf 0/4DSP · surf 0/4TNFAIP3 · surf 0/4ERO1A · surf 0/4S100A4 · surf 0/4PTGS2 · surf 0/4E2F7 · surf 0/4SAMHD1 · surf 0/4CXCL3 · surf 0/4IL1A · surf 0/4CKAP2L · surf 0/4PIK3CD · surf 0/4IFI35 · surf 0/4PARP12 · surf 0/4IFIT3 · surf 0/4HSPA1A · surf 0/4DTX3L · surf 0/4PARP9 · surf 0/4SAMD9L · surf 0/4MXD1 · surf 0/4TUFT1 · surf 0/4TCF21 · surf 0/4LINC01133 · surf 0/4PDGFB · surf 0/4DDX60 · surf 0/4HID1 · surf 0/4KHDRBS3 · surf 0/4USP18 · surf 0/4MYBL1 · surf 0/4ADAP1 · surf 0/4SERPINA9 · surf 0/4IL24 · surf 0/4EPB41L4B · surf 0/4TIMP4 · surf 0/4CA13 · surf 0/4PCSK6 · surf 0/4BATF2 · surf 0/4CCL20 · surf 0/4BEX2 · surf 0/4KRT14 · surf 0/4IL1RN · surf 0/4
No flow literature — custom antibody needed (106)
MARCHF4 · surf 1/4SEC11C · surf 1/4NRK · surf 1/4HRK · surf 1/4SELENOS · surf 1/4KCNK6 · surf 1/4MYRF · surf 1/4SLC22A31 · surf 1/4SYT14 · surf 1/4DNAJC1 · surf 1/4DNAJC22 · surf 1/4TM6SF1 · surf 1/4SLC9A3R2 · surf 0/4DNAJB11 · surf 0/4DNAJB9 · surf 0/4HELZ2 · surf 0/4FICD · surf 0/4DAW1 · surf 0/4SH2D5 · surf 0/4SAMD9 · surf 0/4MEI1 · surf 0/4SFTA1P · surf 0/4CCIN · surf 0/4LINC00942 · surf 0/4SFN · surf 0/4gene_249675 · surf 0/4XDH · surf 0/4PDE2A-AS2 · surf 0/4gene_287112 · surf 0/4VGF · surf 0/4LINC01679 · surf 0/4OLAH · surf 0/4IL36B · surf 0/4KLHL30-AS1 · surf 0/4MYLK2 · surf 0/4DPY19L2P1 · surf 0/4LINC00536 · surf 0/4HAPLN1 · surf 0/4SDF2L1 · surf 0/4PLEK2 · surf 0/4CCN3 · surf 0/4MX2 · surf 0/4EML2-AS1 · surf 0/4DGKI · surf 0/4FRMPD4 · surf 0/4KLHDC7B · surf 0/4PKP1 · surf 0/4PSD4 · surf 0/4gene_295473 · surf 0/4TRIM58 · surf 0/4GREM1-AS1 · surf 0/4KCTD14 · surf 0/4FAM222A · surf 0/4DGKG · surf 0/4HKDC1 · surf 0/4SIK1B · surf 0/4KIF17 · surf 0/4LINC02257 · surf 0/4MYO7B · surf 0/4PADI1 · surf 0/4FSTL3 · surf 0/4GNPNAT1 · surf 0/4WNK4 · surf 0/4C6orf132 · surf 0/4SPECC1 · surf 0/4COL4A4 · surf 0/4OSGIN1 · surf 0/4DYRK3 · surf 0/4PDLIM3 · surf 0/4AK4 · surf 0/4C5orf46 · surf 0/4PRR5L · surf 0/4FHOD3 · surf 0/4FNDC1 · surf 0/4LARP1B · surf 0/4ALS2CL · surf 0/4PSG5 · surf 0/4BAIAP2L2 · surf 0/4NOCT · surf 0/4NAV2-AS6 · surf 0/4NMRAL2P · surf 0/4IQCG · surf 0/4ERO1B · surf 0/4STXBP6 · surf 0/4gene_254710 · surf 0/4PPM1H · surf 0/4ARFGEF3 · surf 0/4PCBP3 · surf 0/4TBX5-AS1 · surf 0/4ZNF296 · surf 0/4gene_295475 · surf 0/4ZNF107 · surf 0/4SPAG1 · surf 0/4gene_274574 · surf 0/4CFAP45 · surf 0/4gene_269182 · surf 0/4gene_287015 · surf 0/4LINC00520 · surf 0/4KBTBD11 · surf 0/4ROR1-AS1 · surf 0/4LINC01588 · surf 0/4gene_274070 · surf 0/4INSC · surf 0/4LINC01444 · surf 0/4MYO18B · surf 0/4TENM3-AS1 · surf 0/4
Reagents are AI-extracted from the Methods of the linked papers; clone/catalog should be verified against the source and vendor before ordering.